Dsrna j2
WebDescription: Anti-dsRNA monoclonal antibody J2 is the gold standard for the detection of dsRNA both in cultured cells and fixed paraffin-embedded histological samples. … dsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6).
Dsrna j2
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WebThe monoclonal anti-dsRNA antibody J2 binds double-stranded RNAs (dsRNA) in an apparently sequence-nonspecific way. The mAb only recognizes antigens with double … Web10 gen 2013 · The J2 monoclonal mouse antibody directed against dsRNA allows the detection of various dsRNA viruses independently of their sequences , . To gauge its utility for LRV detection, it was first tested on …
Web3 feb 2024 · By using the dsRNA-specific antibody J2 for intracellular staining and FACS analysis we assessed the level of dsRNA in MHV-A59- and MHV H277A-infected wild-type and IFNAR-deficient macrophages at 4, 6, 9, and 12 h.p.i.. At 4 h.p.i. dsRNA was not yet convincingly detectable in both MHV-A59- and MHV H277A-infected wild-type … WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic …
WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine …
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WebThe J2 anti-dsRNA IgG2a monoclonal antibody has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, ... the a cardWebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … the aca obamacare createdWebMouse monoclonal antibody J2 recognises double-stranded RNA (dsRNA) provided that the length of the helix is greater than or equal to 40 bp. dsRNA-recognition is independent of … the a careerWebAlternative Name(s) of Target: double-stranded RNA Immunogen: The original J2 antibody was raised by immunising mice with L-dsRNA. Specificity: This antibody binds to dsRNA > 40 bp in length. Application Notes: This recombinant mouse monoclonal antibody, rJ2, is useful for detecting double stranded RNA of greater than 40 bp in length which is … thea carolWebThe J2 anti-dsRNA IgG2a monoclonal antibody has become the gold standard in dsRNA detection. It was used initially for the study of plant viruses, but since the seminal paper of Weber et al. in 2006, where J2 was used to show that all the positive strand RNA viruses tested produced copious amounts of dsRNA in infected cells, ... thea caroline kriminologWebFunctions of long, cellular dsRNAs are poorly understood, although deficiencies in adenosine deaminases that act on RNA, or ADARs, promote their recognition as viral … thea carola wiedWeb22 gen 2014 · The J2 dsRNA monoclonal antibody was previously used to detect viral dsRNA by dsRNA immunoblotting, immunohistochemistry, ELISA, and immunoprecipitation in eukaryotic cells (13–20). In this study, we used the J2 dsRNA antibody in an immunoprecipitation assay to enrich dsRNA from total RNA extracted from E. coli. thea carroll